Inhibition of cholesterol side chain cleavage by active site directed antibody to corpus luteum cytochrome P-450.
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چکیده
منابع مشابه
Inhibition of cholesterol side chain cleavage by active site directed antibody to corpus luteum cytochrome P-450.
Goat antibody IgG produced against bovine corpus luteum mitochondrial cytochrome P-450 (P-450scc) associated with cholesterol side chain cleavage (CSCC) was used to compare immunological characteristics of mitochondrial cytochrome P-450s from the bovine adrenal cortex (BAM), bovine corpus luteum (BCLM), and human placenta (HPM). In Ouchterlony double diffusion, anti-P450scc produced a single ba...
متن کاملRegulation of intramitochondrial cholesterol transfer to side-chain cleavage cytochrome P-450 in rat adrenal gland.
Rat adrenal mitochondria accumulated cholesterol during ether stress in vivo when side-chain cleavage was inhibited by aminoglutethimide (control = 14.6 vs. aminoglutethimide = 26.5 micrograms of cholesterol per mg of protein). This accumulation was insensitive to simultaneous administration of cycloheximide (24.2 micrograms/mg), but side chain cleavage in the mitochondria was greatly decreased...
متن کاملCytochrome P-450 from Bovine Adrenocortical Mitochondria: an Enzyme for the Side Chain Cleavage of Cholesterol
One cytochrome P-450 has been purified from bovine adrenocortical mitochondria to near homogeneity. The enzyme catalyzes the conversion of cholesterol and 20~ hydroxycholesterol to pregnenolone (side chain cleavage) and shows traces of 11/3-hydroxylase activity. The conversion of cholesterol to pregnenolone occurs without demonstrable accumulation of biosynthetic intermediates, i.e. hydroxylate...
متن کاملCytochrome P-450 from Bovine Adrenocortical Mitochondria: an Enzyme for the Side Chain Cleavage of Cholesterol
One cytochrome P-450 has been purified from bovine adrenocortical mitochondria to near homogeneity. The enzyme catalyzes the conversion of cholesterol and 20~ hydroxycholesterol to pregnenolone (side chain cleavage) and shows traces of 11/3-hydroxylase activity. The conversion of cholesterol to pregnenolone occurs without demonstrable accumulation of biosynthetic intermediates, i.e. hydroxylate...
متن کاملAdrenocortical cytochrome P-450 side chain cleavage. Preparation of membrane-bound side chain cleavage system from purified components.
Soluble cytochrome P-450 from bovine adrenocortical mitochrondria, capable of side chain cleavage, can be incorporated into membranes prepared by dispersion of phospholipids in aqueous buffer when cholate is added to the membrane suspension. In addition, the complete protein side chain cleavae system (i.e. including the ancillary proteins adrenodoxin and adrenodoxin reductase and the substrate ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1982
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)34908-1